New Product Highlights from Gatan




Contact: info@gatan.com

GATAN INC.
Corporate Headquarters
5794 W. Las Positas Blvd.
Pleasanton, CA 94588
Tel: (925) 463-0200
Fax: (925) 463-0204

Website: www.gatan.com

Gatan designs and manufactures instruments and products for electron microscopes that enable and advance EM applications. Gatan is the recognized leader in the industry and our products set the industry standards. Our engineers understand the application criteria of our customers and provide them with the right solutions. GATAN GETS IT!

Featured Product: 3View™, Serial block face imaging, a revolution in 3D Microscopy



Image showing a neuropil area  through a mouse barrel cortex (neuron shown in blue and synapses shown in red) processed for EM and generated using Gatan’s 3View™ SBFSEM microscopy system. Eight hundred perfectly aligned image slices of 50 nm were acquired. Gatan’s DigitalMicrograph™ 3D Visualization tool was used to show the Z projection of the 800 slices. Manual segmentation using Reconstruct (GNU General Public License version 2) software generated a 3D reconstruction of the neuronal cell including the dendrites and axon. The inset image shows an identified synapse between a dendrite of the reconstructed neuron and the axon labeled for parvalbumine (pre-embedding immunochemistry with biotinilated antibody). Sample courtesy of Dr. Graham Knott, DBCM, University of Lausanne.

Block face imaging in the SEM: 3View™ uses a specially designed ultra-microtome operating in situ within a variable pressure, Field Emission SEM, and allows automated acquisition of 3D ultrastructure by sequentially imaging the freshly cut, resin embedded block face.
Perfect image stack alignment: Due to the technique of serially imaging the block face rather than individual sections, and because of the stability of 3View's stage, there is excellent registration between all images within an image stack. This allows simple exporting of the image files for 3-D rendering by 3rd party software, without the need for tedious post-processing.
High resolution results: 3View’s unique design allows a relatively short SEM working distance to stay exactly constant throughout a cutting sequence. Furthermore, a finely focused electron probe and small interaction volume enables a spatial resolution over 1 order of magnitude superior to that of confocal microscopy, and approaching the nanometer scale. As there is no depth probing, this 3D resolution is maintained throughout the sectioning depth.

For more information, please visit: http://www.gatan.com/sem/3view.php